ZnO 掺杂碳量子点的流动注射化学发光法测定甲硝唑

Determination of metronidazole by a flow-injection chemiluminescence method using ZnO-doped carbon quantum dots

摘要: 以活性炭为碳源,采用化学氧化结合 ZnO 包覆的方法制备新结构碳量子点,其稳定性好,荧光量子产率高。结果表明,甲硝唑对鲁米诺鄄高锰酸钾体系具有增敏作用,同时能有效猝灭碳量子点的荧光,以此建立化学发光法检测甲硝唑片含量。在最佳条件下,化学发光强度与甲硝唑的浓度呈良好的线性,其线性为 7. 5×10 ^-4 ~2. 5×10 ^-8 g/ mL,线性相关系数 r=0. 9996, 检测限 C_L=1. 08×10^-10 g/ mL。

Abstract: ZnO-doped carbon quantum dots (CQDs) were prepared by the chemical oxidation of activated carbon followed by zinc acetate impregnation and precipitation. The ZnO-doped CQDs had a stable fluorescence and a high fluorescence quantum yield. The fluorescence of the ZnO-doped CQDs can be quenched by reduction with metronidazole, allowing a flow-injection chemilumi-nescence method to be used to determine the metronidazole content in tablets. Under optimum conditions, there was a good linear relation between the concentration of metronidazole and the intensity of chemiluminiscence. The linear concentration range examined was 7. 5×10 ^-4 -2. 5×10 ^-8 g/ mL with r = 0. 999 6 and a detection limit of 1. 08×10 ^-10 g/ mL.